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KMID : 0880220120500040693
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Journal of Microbiology
2012 Volume.50 No. 4 p.693 ~ p.697
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Biosynthetic pathway for poly(3-Hydroxypropionate) in recombinant Escherichia coli
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Qi Wang
Changshui Liu
Mo Xian
Yongguang Zhang
Guang Zhao
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Abstract
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Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexusaurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.
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KEYWORD
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poly(3-hydroxypropionate), malonyl-CoA reductase, propionyl-coenzyme A synthetase, polyhydroxyalkanoate synthase, recombinant E. coli
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